Inhaltsverzeichnis
- Glass Spectrophotometer Cuvettes, For Chemical Laboratory
- Micro Cell With Frosted Walls And Lid Type 18
- Receive The Latest Promotions And Special Offers
Scratches on the sides of the cuvette the light passes through scatter light and cause errors. A rubber or plastic rack protects the cuvette from accidentally hitting and being scratched by the machine casing. Cuvettes to be used in circular dichroism experiments should never be mechanically stressed, as the stress will induce birefringence in the quartz and affect measurements. Nest Scientific Standard and UV Cuvettes have a 10mm optical path length and are compatible with most spectrophotometer makes and models.
- However, factors like transmission range, cost and work environment will influence which material best suits your unique application.
- See below for solvent compatibility and spectral range details.
- Some cuvettes, known as tandem cuvettes, have a glass barrier that extends 2/3 up inside, so that measurements can be taken with two solutions separated, and again when they are mixed.
- Quartz excels at transmitting UV light, and can be used for wavelengths ranging from 190 to 2500 nm.
While 10 mm path lengths are the most widely used, absorption cuvettes come in path lengths ranging from 1 mm to 100 mm, as well. The most common type of cuvette is square, with external dimensions of 12.5 x 12.5 mm. This format accommodates sample volumes from the microliter range (ultra-micro cuvettes) to the milliliter range . Cuvettes are small rectangular glass or quartz containers. They are often designed so that the light beam travels a distance of 1 cm through the contents, but the path length can vary from 1 or 2 mm all the way up to 10 cm.
Glass Spectrophotometer Cuvettes, For Chemical Laboratory
The article examines the optical characteristics of such materials that will help you make cuvette the right choice before proceeding with absorbance measurements. Most often Polymethylmethacrylate and Polystyrene material use to make the plastic cuvettes. Disposable macro cuvettes with 4 optically clear sides for use in flourescence applications. International Crystal Laboratories has quartz & special IR, NIR, UV, & VIS cuvettes for any spectroscopy application. A strong, vibrant research and development group is the lifeblood of all industries. VWR will support you from the latest life science products to the guaranteed purity of organic building blocks...
Micro Cell With Frosted Walls And Lid Type 18
Consider how different sizes and optical materials will influence your processes and choose the appropriate type to get precise results. The cuvette must match your application to yield accurate information on concentration and purity. In order to choose one, the specific use and equipment have to be defined. For example, plastic may not be efficient for UV-range experiments, but it is a cost-effective alternative for all the visible light studies. Plastic cuvettes are often used in fast spectroscopic assays, where high speed is more important than high accuracy. Plastic cuvettes with a usable wavelength range of 380–780nm may be disposed of after use, preventing contamination from re-use.
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Historically, reusable quartz cuvettes were required for measurements in the ultraviolet range, because glass and most plastics absorb ultraviolet light, creating interference. Today there are disposable plastic cuvettes made of specialized plastics that are transparent to ultraviolet light. Glass, plastic and quartz cuvettes are all suitable for measurements made at longer wavelengths, such as in the visible light range.